ARG62492
anti-Filamin antibody [FLMN01 (PM6/317)]
anti-Filamin antibody [FLMN01 (PM6/317)] for ELISA,Immunohistochemistry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Chicken,Guinea pig,Rabbit
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [FLMN01 (PM6/317)] recognizes Filamin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Chk, Gpig, Rb |
| Tested Application | ELISA, IHC, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | FLMN01 (PM6/317) |
| Isotype | IgG1 |
| Target Name | Filamin |
| Immunogen | Platelet filamin |
| Conjugation | Un-conjugated |
| Alternate Names | Endothelial actin-binding protein; ABP-280; XMVD; ABPX; Actin-binding protein 280; FLN1; MNS; OPD1; XLVD; OPD2; OPD; Non-muscle filamin; CSBS; Filamin-A; FLN-A; FLN; NHBP; Filamin-1; FMD; Alpha-filamin; CVD1 |
Application Instructions
| Application Note | WB: 1-2 ug/ml ELISA 1/100 - 1/2000 IHC: 1/10-1/500 IHC-P: 1-2 ug/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | FLNA |
| Gene Full Name | filamin A, alpha |
| Background | The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009] |
| Function | Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. [UniProt] |
| Cellular Localization | Cytoplasm; cell cortex |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 281 kDa |
| PTM | Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA. Phosphorylation extent changes in response to cell activation. Polyubiquitination in the CH1 domain by a SCF-like complex containing ASB2 leads to proteasomal degradation. Prior dissociation from actin may be required to expose the target lysines (PubMed:24052262). Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome (PubMed:26766444). |
Clone References
Cyclin B1/Cdk1 binds and phosphorylates Filamin A and regulates its ability to cross-link actin.
WB / Human
