ARG23188

anti-Firefly Luciferase antibody [LUC-Y]

anti-Firefly Luciferase antibody [LUC-Y] for ELISA,Flow cytometry,IHC-Frozen sections,Western blot and Firefly

Overview

Product Description Mouse Monoclonal antibody [LUC-Y] recognizes Firefly Luciferase
Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
Tested Reactivity Firef
Tested Application ELISA, FACS, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone LUC-Y
Isotype IgG1
Target Name Firefly Luciferase
Antigen Species Firefly
Immunogen Recombinant Firefly Luciferase expressed in E. coli.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:500 - 1:5000
FACS10 ug/ml
IHC-FrAssay-dependent
WB1:100
Application Note FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
WB: This product detects a band of approximately 55 - 60 kDa with a sample of 1 µg of purified luciferase from Photinus pyralis.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.