ARG23188
anti-Firefly Luciferase antibody [LUC-Y]
anti-Firefly Luciferase antibody [LUC-Y] for ELISA,Flow cytometry,IHC-Frozen sections,Western blot and Firefly
Overview
Product Description | Mouse Monoclonal antibody [LUC-Y] recognizes Firefly Luciferase Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues. |
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Tested Reactivity | Firef |
Tested Application | ELISA, FACS, IHC-Fr, WB |
Host | Mouse |
Clonality | Monoclonal |
Clone | LUC-Y |
Isotype | IgG1 |
Target Name | Firefly Luciferase |
Antigen Species | Firefly |
Immunogen | Recombinant Firefly Luciferase expressed in E. coli. |
Conjugation | Un-conjugated |
Application Instructions
Application Suggestion |
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Application Note | FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. WB: This product detects a band of approximately 55 - 60 kDa with a sample of 1 µg of purified luciferase from Photinus pyralis. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purification with Protein A. |
Buffer | PBS and 0.09% Sodium azide. |
Preservative | 0.09% Sodium azide |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |