ARG55493

anti-Flotillin 1 antibody

anti-Flotillin 1 antibody for ICC/IF,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Signaling Transduction antibody
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1

Overview

Product Description

Rabbit Polyclonal antibody recognizes Flotillin 1

Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Flotillin 1
Antigen Species Human
Immunogen Recombinant protein of Human FLOT1 (NP_005794.1)
Conjugation Un-conjugated
Alternate Names Flotillin-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10211 Human FLOT1

GeneID: 14251 Mouse FLOT1

Swiss-port # O08917 Mouse Flotillin-1

Swiss-port # O75955 Human Flotillin-1

Gene Symbol FLOT1
Gene Full Name flotillin 1
Background Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq, Jul 2008]
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 47 kDa

Customer's Feedback

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Review for anti-Flotillin 1 antibody

Application:WB

Sample:HeLa and 293T

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC