ARG54079
anti-Fyn antibody
anti-Fyn antibody for Western blot,ICC/IF and Human,Monkey,Rat,Mouse
Cancer antibody; Neuroscience antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes FYN |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | Fyn |
| Antigen Species | Human |
| Immunogen | Purified recombinant human Fyn protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | p59-FYN; Tyrosine-protein kinase Fyn; Src-like kinase; Proto-oncogene c-Fyn; p59-Fyn; Proto-oncogene Syn; SYN; SLK; EC 2.7.10.2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 59 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 10 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | FYN |
| Gene Full Name | FYN proto-oncogene, Src family tyrosine kinase |
| Background | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival,cell adhesion,integrin-mediated signaling,cytoskeletal remodeling,cell motility,immune response and axon guidance.Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain.Following activation by PKA,the protein subsequently associates with PTK2/FAK1,allowing PTK2/FAK1 phosphorylation,activation and targeting to focal adhesions.Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT.Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage.Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1,KIRREL and TRPC6.Plays a role in neural processes by phosphorylating DPYSL2,a multifunctional adapter protein within the central nervous system,ARHGAP32,a regulator for Rho family GTPases implicated in various neural functions, and SNCA,a small pre-synaptic protein.Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation.Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1,thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts.CSK maintains LCK and FYN in an inactive form.Promotes CD28-induced phosphorylation of VAV1. |
| Function | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking. |
| Highlight | Related Antibody Duos and Panels: ARG30169 Src Family Protein Tyrosine Kinases Antibody Panel Related products: Fyn antibodies; Fyn Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cancer antibody; Neuroscience antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody |
| Calculated MW | 61 kDa |
| PTM | Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (By similarity). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Palmitoylation at Cys-3 and Cys-6 regulates subcellular location. |
