ARG56677

anti-G-CSF antibody

anti-G-CSF antibody for ELISA,Neutralizing,Western blot and Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes G-CSF
Tested Reactivity Ms
Tested Application ELISA, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name G-CSF
Antigen Species Mouse
Immunogen E.coli derived Recombinant Mouse G-CSF.
(MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA)
Conjugation Un-conjugated
Alternate Names Granulocyte colony-stimulating factor; Lenograstim; C17orf33; GCSF; G-CSF; Filgrastim; Pluripoietin; CSF3OS

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56787 as a detection antibody
Neut0.005 - 0.010 µg/ml (To yield [ND50] of the biological activity of mG - CSF (0.5ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12985 Mouse CSF3

Swiss-port # P09920 Mouse Granulocyte colony-stimulating factor

Gene Symbol Csf3
Gene Full Name colony stimulating factor 3 (granulocyte)
Background The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. [UniProt]
Calculated MW 22 kDa
PTM O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).