ARG40829

anti-GAD2 / GAD65 antibody

anti-GAD2 / GAD65 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GAD2 / GAD65
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GAD2 / GAD65
Antigen Species Human
Immunogen Recombinant protein corresponding to K84-L182 of Human GAD65.
Conjugation Un-conjugated
Alternate Names Glutamate decarboxylase 65 kDa isoform; EC 4.1.1.15; GAD65; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14417 Mouse GAD2

GeneID: 24380 Rat GAD2

GeneID: 2572 Human GAD2

Gene Symbol GAD2
Gene Full Name glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Background This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
Function Catalyzes the production of GABA. [UniProt]
Cellular Localization Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. [UniProt]
Calculated MW 65 kDa
PTM Phosphorylated; which does not affect kinetic parameters or subcellular location.

Palmitoylated; which is required for presynaptic clustering. [UniProt]