ARG40855
anti-GAD2 / GAD65 antibody
anti-GAD2 / GAD65 antibody for Western blot and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody recognizes GAD2 / GAD65 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | GAD2 / GAD65 |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human GAD2 protein fragments. |
| Conjugation | Un-conjugated |
| Alternate Names | Glutamate decarboxylase 65 kDa isoform; EC 4.1.1.15; GAD65; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol. |
| Preservative | 0.03% Proclin 300 |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GAD2 |
| Gene Full Name | glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) |
| Background | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| Function | Catalyzes the production of GABA. [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. [UniProt] |
| Calculated MW | 65 kDa |
| PTM | Phosphorylated; which does not affect kinetic parameters or subcellular location. Palmitoylated; which is required for presynaptic clustering. [UniProt] |
