ARG63756

anti-GAD2 / GAD65 antibody

anti-GAD2 / GAD65 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Metabolism antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes GAD2 / GAD65
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise both reported variants.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GAD2 / GAD65
Antigen Species Human
Immunogen C-TLEDNEERMSRLSK
Conjugation Un-conjugated
Alternate Names Glutamate decarboxylase 65 kDa isoform; EC 4.1.1.15; GAD65; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay - dependent
WB0.5 - 1.5 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2572 Human GAD2

Swiss-port # Q05329 Human Glutamate decarboxylase 2

Background This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
Research Area Metabolism antibody; Neuroscience antibody
Calculated MW 65 kDa
PTM Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.