ARG64797

anti-GAD67 antibody

anti-GAD67 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Immune System antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes GAD67
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GAD67
Antigen Species Human
Immunogen C-PDSPQRREKLHK
Conjugation Un-conjugated
Alternate Names EC 4.1.1.15; Glutamate decarboxylase 67 kDa isoform; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD; SCP; GAD-67; CPSQ1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay - dependent
WB0.5 - 1.5 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2571 Human GAD1

Swiss-port # Q99259 Human Glutamate decarboxylase 1

Background This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
Research Area Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 67 kDa