ARG54931

anti-GADD45 alpha antibody

anti-GADD45 alpha antibody for Flow cytometry,Western blot and Human

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes GADD45A
Tested Reactivity Hu
Predict Reactivity Bov
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GADD45 alpha
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human GADD45 alpha.
Conjugation Un-conjugated
Alternate Names Growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT1; GADD45; DNA damage-inducible transcript 1 protein; DDIT-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Y79

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1647 Human GADD45A

Swiss-port # P24522 Human Growth arrest and DNA damage-inducible protein GADD45 alpha

Gene Symbol GADD45A
Gene Full Name growth arrest and DNA-damage-inducible, alpha
Background This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]
Function In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. [UniProt]
Cellular Localization Nucleus.
Research Area Gene Regulation antibody
Calculated MW 18 kDa