ARG55113
anti-GATA3 antibody
anti-GATA3 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GATA3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GATA3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (17 aa) within aa. 340-390 of Human GATA3 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; HDR; HDRS |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human Brain Tissue Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P23771 Human Trans-acting T-cell-specific transcription factor GATA-3 Swiss-port # P23772 Mouse Trans-acting T-cell-specific transcription factor GATA-3 |
|---|---|
| Gene Symbol | GATA3 |
| Gene Full Name | GATA binding protein 3 |
| Background | GATA3 Antibody: GATA3 is a zinc finger transcription factor that was first recognized as a possible determinant of T cell development. Later studies demonstrated that it is expressed in trophoblast giant cells and regulates trophoblast-specific genes during development. Specifically, GATA3 is selectively expressed in the trophectoderm of the peri-implantation embryo and regulates CDX2, which is required for the transcriptional repression of the POU5F1/Oct4 and NANOG genes and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Decreased or null-expression expression of GATA3 has also been suggested to play a major role in the development and progression of luminal breast cancer. |
| Function | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. [UniProt] |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody |
| Calculated MW | 48 kDa |
