ARG58109
anti-GCLC antibody
anti-GCLC antibody for Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GCLC |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GCLC |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-252 of Human GCLC (NP_001489.1). |
| Conjugation | Un-conjugated |
| Alternate Names | GCS heavy chain; Gamma-glutamylcysteine synthetase; Gamma-ECS; GCL; GLCL; GCS; Glutamate--cysteine ligase catalytic subunit; EC 6.3.2.2; GLCLC |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Mouse liver | ||||
| Observed Size | ~ 75 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P19468 Rat Glutamate--cysteine ligase catalytic subunit Swiss-port # P97494 Mouse Glutamate--cysteine ligase catalytic subunit |
|---|---|
| Gene Symbol | GCLC |
| Gene Full Name | glutamate-cysteine ligase, catalytic subunit |
| Background | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010] |
| Calculated MW | 73 kDa |
