ARG59635

anti-GCNT1 antibody

anti-GCNT1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes GCNT1
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GCNT1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 88-117 of Human GCNT1.
Conjugation Un-conjugated
Alternate Names NAGCT2; Core 2 GNT; G6NT; C2GNT1; Core 2-branching enzyme; Core2-GlcNAc-transferase; NACGT2; C2GNT; EC 2.4.1.102; C2GNT-L; Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2650 Human GCNT1

Swiss-port # Q02742 Human Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminylt

Gene Symbol GCNT1
Gene Full Name glucosaminyl (N-acetyl) transferase 1, core 2
Background This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Function Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine moiety onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan. Mucin-type core 2 O-glycans play an important role in leukocyte extravasation as they serve as scaffolds for the display of the selectin ligand sialyl Lewis X by leukocytes. [UniProt]
Cellular Localization Golgi apparatus membrane; Single-pass type II membrane protein. Note=Also detected in the trans-Golgi network. [UniProt]
Calculated MW 50 kDa