ARG53934
anti-GCPII / PSMA antibody [GCP-05]
anti-GCPII / PSMA antibody [GCP-05] for CyTOF®-candidate,Flow cytometry,ICC/IF,Immunoprecipitation and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [GCP-05] recognizes GCPII / PSMA |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, ICC/IF, IP |
| Specificity | The clone GCP-05 recognizes extracellular domain of glutamate carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | GCP-05 |
| Isotype | IgG1 |
| Target Name | GCPII / PSMA |
| Antigen Species | Human |
| Immunogen | amino acids 44-750 of human GCPII |
| Conjugation | Un-conjugated |
| Alternate Names | FGCP; GCP2; PSM; Glutamate carboxypeptidase II; PSMA; NAALADase I; Membrane glutamate carboxypeptidase; Prostate-specific membrane antigen; FOLH; N-acetylated-alpha-linked acidic dipeptidase I; GCPII; Glutamate carboxypeptidase 2; NAALAdase; NAALAD1; Folate hydrolase 1; EC 3.4.17.21; Folylpoly-gamma-glutamate carboxypeptidase; mGCP; Cell growth-inhibiting gene 27 protein; Pteroylpoly-gamma-glutamate carboxypeptidase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FOLH1 |
| Gene Full Name | folate hydrolase (prostate-specific membrane antigen) 1 |
| Background | Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation. |
| Function | Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. [UniProt] |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 84 kDa |
| PTM | The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis. The N-terminus is blocked. |
Clone References
Gold nano-popcorn-based targeted diagnosis, nanotherapy treatment, and in situ monitoring of photothermal therapy response of prostate cancer cells using surface-enhanced Raman spectroscopy.
ICC/IF / Human
Expression of glutamate carboxypeptidase II in human brain.
Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding.
Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.
Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II.
