ARG63901

anti-GEF5 antibody

anti-GEF5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes GEF5
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GEF5
Antigen Species Human
Immunogen C-DKEIDQNSQQEE
Conjugation Un-conjugated
Alternate Names P60; TIM1; Oncogene TIM; Rho guanine nucleotide exchange factor 5; TIM; GEF5; Ephexin-3; p60 TIM; Transforming immortalized mammary oncogene; Guanine nucleotide regulatory protein TIM

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7984 Human ARHGEF5

Swiss-port # Q12774 Human Rho guanine nucleotide exchange factor 5

Background Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 177 kDa
PTM Activation of SRC induces tyrosine phosphorylation of ARHGEF5.