ARG11126

anti-GFAP antibody [5C10]

anti-GFAP antibody [5C10] for Western blot,ICC/IF,IHC-Frozen sections and Human,Mouse,Rat,Pig,Horse

Overview

Product Description Mouse Monoclonal antibody [5C10] recognizes GFAP
Tested Reactivity Hu, Ms, Rat, Hrs, Pig
Predict Reactivity Cow
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 5C10
Isotype IgG1
Target Name GFAP
Antigen Species Pig
Immunogen Purified pig spinal cord GFAP.
Conjugation Un-conjugated
Alternate Names Glial fibrillary acidic protein; ALXDRD; GFAP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
WB1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 50 kDa

Properties

Form Liquid
Purification Purified
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

Gene Symbol GFAP
Gene Full Name glial fibrillary acidic protein
Background This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
Cellular Localization Cytoplasm. Note=Associated with intermediate filaments. [UniProt]
Calculated MW 50 kDa
PTM Phosphorylated by PKN1. [UniProt]