ARG52313

anti-GFAP antibody

anti-GFAP antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Bovine,Cat,Chicken,Human,Mouse,Rabbit,Rat,Rhesus Monkey

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody

Overview

Product Description

Chicken Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP)

Tested Reactivity Hu, Ms, Rat, Bov, Cat, Chk, R. Mk, Rb
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name GFAP
Antigen Species Bovine
Immunogen Recombinant and purified bovine GFAP
Conjugation Un-conjugated
Alternate Names Glial fibrillary acidic protein; ALXDRD; GFAP

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:5000
FACS1:100
ICC/IF1:1000
IHC-Fr1:1000
IHC-P1:1000
WB1:10000
Application Note Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Total IgY fraction
Buffer Total IgY fraction in PBS and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

Gene Symbol GFAP
Gene Full Name glial fibrillary acidic protein
Background GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
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Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
Calculated MW 50 kDa
PTM Phosphorylated by PKN1.