ARG62991

anti-GFAP antibody [GF-01]

anti-GFAP antibody [GF-01] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Cat,Human,Pig

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody

Overview

Product Description Mouse Monoclonal antibody [GF-01] recognizes Glial Fibrillary Acidic Protein (GFAP)
Tested Reactivity Hu, Cat, Pig
Species Does Not React With Ms, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Specificity The clone GF-01 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves.
The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Host Mouse
Clonality Monoclonal
Clone GF-01
Isotype IgG1
Target Name GFAP
Antigen Species Pig
Immunogen Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
Conjugation Un-conjugated
Alternate Names Glial fibrillary acidic protein; ALXDRD; GFAP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 - 10 µg/ml
IHC-P10 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note IHC-P: The clone GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: Human brain (cortex, cerebellum)

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2670 Human GFAP

GeneID: 396562 Pig GFAP

Swiss-port # P14136 Human Glial fibrillary acidic protein

Background GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
Highlight Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Astrocyte-to-neuron conversion for Parkinson's disease treatment
Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
Calculated MW 50 kDa
PTM Phosphorylated by PKN1.