ARG10122

anti-GFAP antibody [GF5]

anti-GFAP antibody [GF5] for ELISA,ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
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Overview

Product Description

Mouse Monoclonal antibody [GF5] recognizes Glial Fibrillary Acidic Protein (GFAP)

Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC-Fr, WB
Specificity This antibody is specific for Human GFAP. There is no cross-reactivity with other neurospecific proteins.
Host Mouse
Clonality Monoclonal
Clone GF5
Isotype IgG2b
Target Name GFAP
Antigen Species Human
Immunogen 43-45 kD band corresponding to GFAP in immunoblotting of extract from human brain and spinal cord.
Conjugation Un-conjugated
Alternate Names Glial fibrillary acidic protein; ALXDRD; GFAP

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1:100 - 1:1000
IHC-Fr1:100 - 1:1000
WB1:250 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

Gene Symbol GFAP
Gene Full Name glial fibrillary acidic protein
Background GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30304 Astrocyte Maturation / Muller Cell Marker Antibody Duo (GFAP, Vimentin)
ARG30315 Brain Injury IHC Marker Antibody Duo (GFAP, MMP9)
Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
Calculated MW 50 kDa
PTM Phosphorylated by PKN1.

Customer's Feedback

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Review for anti-GFAP antibody [GF5]

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Review for anti-GFAP antibody [GF5]

Application:IF/ICC

Sample:Rat astrocyte primary cell

Fixation Buffer:PFA

Primary Antibody Dilution Factor:1:200

Specific References

Restraint stress promotes lung cancer xenograft growth via the IL2-Ras-Erk pathway

WB / Mouse

Ke Xu et al.
Research Square.,  (2023)

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Effects of Chronic Restraint Stress on Apoptosis of Amygdala Cells in Rats

IHC-P / Rat

Li-Ru Dong et al.
Fa Yi Xue Za Zhi.,  (2022)

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Hyperbaric Oxygen Therapy Attenuates Burn-Induced Denervated Muscle Atrophy

IHC-Fr / Rat

Chin-An Chen et al.
Int J Med Sci.,  (2021)

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RIP3/MLKL pathway-regulated necroptosis: A new mechanism of paclitaxel-induced peripheral neuropathy

IHC-Fr / Rat

Dongyang Ma et al.
J Biochem Mol Toxicol.,  (2021)

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Mechanisms of bupivacaine inhibition of glutamate-induced intracellular calcium elevation in primary cultured rat hippocampal astrocytes.

ICC/IF / Rat

Huan-huan Ma et al.
药学学报,  (2020)

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Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain Hypersensitivity in a Rat Model of Inflammatory Pain.

WB, IF, IHC-Fr / Rat

Luo Zhenzhao et al.
Cell Mol Neurobiol.,  (2019)

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