ARG62993

anti-GFP antibody

anti-GFP antibody for ICC/IF,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes GFP
Tested Reactivity Other
Tested Application ICC/IF, IP, WB
Specificity The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GFP
Immunogen EGFP, a native full-length protein.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1 - 3 µg/ml
IP10 - 20 µg/sample
WB0.5 - 1.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from rabbit serum by protein-A afinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7011691 Other gfp

Background Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ=509 nm) when excited (excitation by Blue or UV light, absorption peak λ=395 nm).

GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein.

GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.
Research Area Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Specific References

HSPA13 modulates type I interferon antiviral pathway and NLRP3 inflammasome to restrict dengue virus infection in macrophages

WB / Human

Qiaohua Wang et al.
Int Immunopharmacol.,  (2023)

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