ARG62343

anti-GFP antibody [GF28R]

anti-GFP antibody [GF28R] for Dot blot,ELISA,ICC/IF,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Overview

Product Description Mouse Monoclonal antibody [GF28R] recognizes GFP
Tested Reactivity Other
Tested Application Dot, ELISA, ICC/IF, IP, WB
Specificity Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Host Mouse
Clonality Monoclonal
Clone GF28R
Isotype IgG2b
Target Name GFP
Antigen Species Others
Immunogen GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugates.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ELISAAssay-dependent
ICC/IF1:500-1:2000
IPAssay-dependent
WB1:1000-1:3000
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A Purified
Purification Note Protein A affinity chromatography from mouse ascites fluid.
Buffer 10mM PBS (pH 7.2) and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7011691 Other gfp

Background In cell and molecular biology, the GFP protein (238aa, 26.9kDa) is frequently used as a reporter to analysis the protein expressing. In modified forms of GFP has been used to be biosensors, and many animals have been created GFP expressing model to analysis gene expression and biological function by transfecting GFP expressing gene into organisms or cells by breeding, injection with a viral vector, or cell transformation. In addition, the GFP gene has been transfected and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein. [Modified from wikipedia]
Highlight Related Antibody Duos and Panels:
ARG30001 Tag Internal Control Antibody Duo (GFP, GAPDH)
ARG30209 Fluorescent-Tags Antibody Panel
Related products:
GFP antibodies; GFP ELISA Kits; GFP Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Clone References

Glucocerebrosidase depletion enhances cell-to-cell transmission of α-synuclein.

Bae EJ et al.
Nat Commun.,  (2014)

publication_link

 

hr_line

Domain specificity of MAP3K family members, MLK and Tak1, for JNK signaling in Drosophila.

WB / 

Stronach B et al.
Genetics.,  (2014)

publication_link

 

hr_line

Phosphatidylinositol phosphate 5-kinase Iγi2 in association with Src controls anchorage-independent growth of tumor cells.

ICC/IF / 

Thapa N et al.
J Biol Chem.,  (2013)

publication_link

 

hr_line

Comparative functional analysis of full-length and N-terminal fragments of phytochrome C, D and E in red light-induced signaling.

WB / 

Ádám É et al.
New Phytol.,  (2013)

publication_link

 

hr_line

Vesicular stomatitis virus infects resident cells of the central nervous system and induces replication-dependent inflammatory responses.

WB / 

Chauhan VS et al.
Virology.,  (2010)

publication_link

 

hr_line

Treatment of Parkinson's disease model mice with allogeneic embryonic stem cells: necessity of immunosuppressive treatment for sustained improvement.

IHC / 

Toriumi H et al.
Neurol Res.,  (2009)

publication_link

 

hr_line