ARG44262
anti-GLI1 antibody
anti-GLI1 antibody for Flow cytometry,ICC/IF and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes GLI1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | FACS, ICC/IF |
| Specificity | This antibody is expected to recognize the reported isoforms |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GLI1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human GLI1 (TYSQCPRLEHYGQ) |
| Conjugation | Un-conjugated |
| Alternate Names | GLI; Glioma-associated oncogene; Zinc finger protein GLI1; Oncogene GLI |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GLI1 |
| Gene Full Name | GLI family zinc finger 1 |
| Background | This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Function | Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling (Probable). Isoform 2: Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441). Nucleus. [UniProt] |
| PTM | Phosphorylated in vitro by ULK3. Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1. [UniProt] |
