ARG58847
anti-GLO1 antibody
anti-GLO1 antibody for Western blot,ICC/IF and Human,Mouse
Overview
Product Description | Rabbit Polyclonal antibody recognizes GLO1 |
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Tested Reactivity | Hu, Ms |
Tested Application | ICC/IF, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | GLO1 |
Antigen Species | Human |
Immunogen | Recombinant fusion protein corresponding to aa. 1-184 of Human GLO1 (NP_006699.2). |
Conjugation | Un-conjugated |
Alternate Names | HEL-S-74; Glyoxalase I; Lactoylglutathione lyase; Glx I; Methylglyoxalase; Ketone-aldehyde mutase; GLOD1; Aldoketomutase; S-D-lactoylglutathione methylglyoxal lyase; GLYI; EC 4.4.1.5 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | 22Rv1 | ||||||
Observed Size | 21 kDa |
Properties
Form | Liquid |
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Purification | Affinity purified. |
Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | GLO1 |
Gene Full Name | glyoxalase I |
Background | The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008] |
Function | Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis. [UniProt] |
Calculated MW | 21 kDa |
PTM | Glutathionylation at Cys-139 inhibits enzyme activity. Phosphorylated at Thr-107 in the presence of CaMK2. However, this is a consensus site for phosphorylation by CK2 so phosphorylation may be mediated by CK2 rather than CaMK2. Phosphorylation is induced by TNF and suppresses the TNF-induced transcriptional activity of NF-kappa-B. Exists in a nitric oxide (NO)-modified form. The exact nature of the modification is unknown, but it suppresses the TNF-induced transcriptional activity of NF-kappa-B. [UniProt] |