ARG65167

anti-GLUD1 + GLUD2 antibody

anti-GLUD1 + GLUD2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
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Overview

Product Description Goat Polyclonal antibody recognizes GLUD1 + GLUD2
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Dog
Tested Application ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognize both GLUD1 and GLUD2 proteins based on sequence homology analysis result.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GLUD1 + GLUD2
Antigen Species Human
Immunogen C-ESEEQKRNRVRGILR
Conjugation Un-conjugated
Alternate Names GDH1; GDH 1; Glutamate dehydrogenase 1, mitochondrial; GDH; GLUD; EC 1.4.1.3; Glutamate dehydrogenase 2, mitochondrial; GLUDP1; EC 1.4.1.3; GDH 2; GDH2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay - dependent
IHC-P3 - 5 µg/ml
WB0.3 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14661 Mouse GLUD1

GeneID: 24399 Rat GLUD1

GeneID: 2746 Human GLUD1

Gene Symbol GLUD1
Gene Full Name glutamate dehydrogenase 1
Background This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.[provided by RefSeq, Sep 2009]
Function Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity). [UniProt]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 61 kDa
PTM ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity (By similarity). Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.

Customer's Feedback

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Review for anti-GLUD1 + GLUD2 antibody

Application:WB

Sample:Rat liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-GLUD1 + GLUD2 antibody

Application:WB

Sample:Mouse liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC