ARG42857
anti-GNE antibody
anti-GNE antibody for Western blot,ICC/IF,Immunoprecipitation and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GNE |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GNE |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human GNE. |
| Conjugation | Un-conjugated |
| Alternate Names | DMRV; GLCNE; ManAc kinase; IBM2; NM; Uae1; Uridine diphosphate-N-acetylglucosamine-2-epimerase; hydrolyzing; UDP-GlcNAc-2-epimerase; EC 3.2.1.183; EC 2.7.1.60; Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa | ||||||||
| Observed Size | ~ 75 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O35826 Rat Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase Swiss-port # Q9Y223 Human Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinas |
|---|---|
| Gene Symbol | GNE |
| Gene Full Name | glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase |
| Background | The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 79 kDa |
| PTM | Phosphorylated by PKC. [UniProt] |
