ARG42857

anti-GNE antibody

anti-GNE antibody for Western blot,ICC/IF,Immunoprecipitation and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GNE
Tested Reactivity Hu, Rat
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GNE
Antigen Species Human
Immunogen Synthetic peptide of Human GNE.
Conjugation Un-conjugated
Alternate Names DMRV; GLCNE; ManAc kinase; IBM2; NM; Uae1; Uridine diphosphate-N-acetylglucosamine-2-epimerase; hydrolyzing; UDP-GlcNAc-2-epimerase; EC 3.2.1.183; EC 2.7.1.60; Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50
IP1:20
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 75 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10020 Human GNE

GeneID: 114711 Rat GNE

Swiss-port # O35826 Rat Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase

Swiss-port # Q9Y223 Human Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinas

Gene Symbol GNE
Gene Full Name glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Background The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 79 kDa
PTM Phosphorylated by PKC. [UniProt]