ARG41955

anti-GPX4 antibody

anti-GPX4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Zebrafish

Ferroptosis/Oxytosis Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes GPX4
Tested Reactivity Hu, Ms, Rat, Zfsh
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GPX4
Antigen Species Human
Immunogen Recombinant protein of Human GPX4.
Conjugation Un-conjugated
Alternate Names Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; EC 1.11.1.12; MCSP; PHGPx; GSHPx-4; Glutathione peroxidase 4; GPx-4; snPHGPx; snGPx

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse kidney
Observed Size ~ 17 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer TBS (pH 7.4), 0.05% Sodium azide, 40% Glycerol and 1% BSA.
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol and 1% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2879 Human GPX4

GeneID: 29328 Rat GPX4

GeneID: 625249 Mouse GPX4

Gene Symbol GPX4
Gene Full Name glutathione peroxidase 4
Background This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage (By similarity). [UniProt]
Cellular Localization Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. [UniProt]
Highlight Related products:
GPX4 antibodies; GPX4 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Ferroptosis/Oxytosis Antibody Panel is launched
Therapeutic strategies against PDAC
Research Area Ferroptosis/Oxytosis Study antibody
Calculated MW 22 kDa