ARG64038

anti-GPX4 antibody

anti-GPX4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Ferroptosis/Oxytosis Study antibody

Overview

Product Description Goat Polyclonal antibody recognizes GPX4
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Pig
Tested Application IHC-P, IP, WB
Specificity This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GPX4
Antigen Species Human
Immunogen C-EEPLVIEKDLPHY
Conjugation Un-conjugated
Alternate Names Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; EC 1.11.1.12; MCSP; PHGPx; GSHPx-4; Glutathione peroxidase 4; GPx-4; snPHGPx; snGPx

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.3 - 1 µg/ml
IPAssay - dependent
WB0.1 - 0.3 µg/ml
Application Note IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2879 Human GPX4

Swiss-port # P36969 Human Phospholipid hydroperoxide glutathione peroxidase, mitochondrial

Background Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq, Jul 2008]
Highlight Related products:
GPX4 antibodies; GPX4 Duos / Panels; Anti-Goat IgG secondary antibodies;
Related news:
Ferroptosis/Oxytosis Antibody Panel is launched
Therapeutic strategies against PDAC
Research Area Ferroptosis/Oxytosis Study antibody
Calculated MW 22 kDa