ARG63175

anti-GRB2 antibody

anti-GRB2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes GRB2
Tested Reactivity Hu, Ms, Rat, Pig
Predict Reactivity Cow, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GRB2
Antigen Species Human
Immunogen C-PRNYVTPVNRNV
Conjugation Un-conjugated
Alternate Names SH2/SH3 adapter GRB2; EGFRBP-GRB2; Adapter protein GRB2; MST084; MSTP084; Grb3-3; ASH; Growth factor receptor-bound protein 2; NCKAP2; Protein Ash

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
WB0.05 - 0.15 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14784 Mouse GRB2

GeneID: 2885 Human GRB2

GeneID: 81504 Rat GRB2

Background The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 25 kDa