ARG58348
anti-GW182 antibody
anti-GW182 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GW182 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GW182 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence within aa. 950-1050 of Human GW182 (NP_055309.2). |
| Conjugation | Un-conjugated |
| Alternate Names | GW182; Trinucleotide repeat-containing gene 6A protein; EMSY interactor protein; GW182 autoantigen; Glycine-tryptophan protein of 182 kDa; GW1; CAG repeat protein 26; CAGH26; Protein GW1; TNRC6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Rat brain | ||||||
| Observed Size | 180 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3UHK8 Mouse Trinucleotide repeat-containing gene 6A protein Swiss-port # Q8NDV7 Human Trinucleotide repeat-containing gene 6A protein |
|---|---|
| Gene Symbol | TNRC6A |
| Gene Full Name | trinucleotide repeat containing 6A |
| Background | This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008] |
| Function | Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes. [UniProt] |
| Cellular Localization | Cytoplasm, P-body. [UniProt] |
| Calculated MW | 210 kDa |
