ARG53062

anti-Galectin 1 antibody [SP247]

anti-Galectin 1 antibody [SP247] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Cell Death antibody
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Overview

Product Description

Rabbit Monoclonal antibody [SP247] recognizes Galectin 1

Tested Reactivity Hu, Ms
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP247
Isotype IgG
Target Name Galectin 1
Antigen Species Human
Immunogen Synthetic peptide near the C-terminus of the Human Galectin 1 protein.
Conjugation Un-conjugated
Alternate Names HBL; Gal-1; GAL1; HPL; Lectin galactoside-binding soluble 1; Galectin-1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; GBP; Beta-galactoside-binding lectin L-14-I; Putative MAPK-activating protein PM12; 14 kDa lectin; Galaptin; S-Lac lectin 1; HLBP14

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-Dependent
IHC-P1:100
WB1:100 - 1:250
Application Note IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Incubation Time: 10 at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Placenta, Tonsil, NIH/3T3 Cell Lysate

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16852 Mouse LGALS1

GeneID: 3956 Human LGALS1

Swiss-port # P09382 Human Galectin-1

Swiss-port # P16045 Mouse Galectin-1

Background Galectin-1 (gal-1) is expressed intracellularly and extracellularly by various normal and pathological tissues. It shows a wide range of biological activity such as apoptosis, cell proliferation and cell differentiation. Gal-1 is overexpressed in endothelial cells of different human tumors, tumor cells, and extracellular matrix. It binds its ligands such as beta-galactoside and a wide array of complex carbohydrates to regulate immune responses.
Cellular Localization Cytoplasm, Extracellular Matrix, Nucleus
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 15 kDa

Customer's Feedback

nuts_pic      Good

Review for anti-Galectin 1 antibody [SP247]

Application:WB

Sample:Rat kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Galectin 1 antibody [SP247]

Application:WB

Sample:Mouse kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC