ARG43576

anti-Galectin 3 antibody

anti-Galectin 3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Flow cytometry,Immunoprecipitation and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Galectin 3.
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Galectin 3
Antigen Species Human
Immunogen Purified recombinant protein corresponding to human Galectin 3.
Protein Full Name Galectin-3
Alternate Names L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:20 - 1:200
ICC/IF1:10 - 1:100
IHC-P1:50 - 1:200
IP1:10 - 1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3958 Human LGALS3

Swiss-port # P17931 Human Galectin-3

Gene Symbol LGALS3
Gene Full Name lectin, galactoside-binding, soluble, 3
Background This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt]