ARG56927

anti-Geminin antibody [2H7]

anti-Geminin antibody [2H7] for Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [2H7] recognizes Geminin
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 2H7
Isotype IgG1, kappa
Target Name Geminin
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-209 of Human Geminin.
Conjugation Un-conjugated
Alternate Names Geminin; Gem

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51053 Human GMNN

Swiss-port # O75496 Human Geminin

Gene Symbol GMNN
Gene Full Name geminin, DNA replication inhibitor
Background This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]
Function Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control. [UniProt]
Calculated MW 24 kDa
PTM Phosphorylated during mitosis. Phosphorylation at Ser-184 by CK2 results in enhanced binding to Hox proteins and more potent inhibitory effect on Hox transcriptional activity.