ARG57501

anti-Glutamine Synthetase antibody [8D7]

anti-Glutamine Synthetase antibody [8D7] for ICC/IF,Western blot and Human,Mouse

Overview

Product Description

Mouse Monoclonal antibody [8D7] recognizes Glutamine Synthetase

Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 8D7
Isotype IgG1, kappa
Target Name Glutamine Synthetase
Antigen Species Human
Immunogen Recombinant Human Glutamine Synthetase (aa. 1-373) purified from E. coli.
Conjugation Un-conjugated
Alternate Names GS; PIG43; Glutamate decarboxylase; EC 6.3.1.2; PIG59; Glutamine synthetase; Glutamate--ammonia ligase; GLNS; EC 4.1.1.15

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14645 Mouse GLUL

GeneID: 2752 Human GLUL

Swiss-port # P15104 Human Glutamine synthetase

Swiss-port # P15105 Mouse Glutamine synthetase

Gene Symbol GLUL
Gene Full Name glutamate-ammonia ligase
Background The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Function This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. [UniProt]
Calculated MW 42 kDa
PTM Ubiquitinated by ZNRF1.