ARG52321
anti-Glycine Receptor antibody
anti-Glycine Receptor antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Glycine Receptor |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Glycine Receptor |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Glycine receptor 48 kDa subunit; STHE; HKPX1; Glycine receptor strychnine-binding subunit; Glycine receptor subunit alpha-1 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~48k α1- and α2-subunits of the glycine receptor in Western blots of Rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other glycine receptor subunits. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity Purified |
| Buffer | lyophilized |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GLRA1 |
| Gene Full Name | glycine receptor, alpha 1 |
| Background | Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002). |
| Research Area | Neuroscience antibody |
| Calculated MW | 53 kDa |
