ARG62497

anti-Glycophorin C antibody [Ret40f]

anti-Glycophorin C antibody [Ret40f] for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human

Cell Biology and Cellular Response antibody

Overview

Product Description Mouse Monoclonal antibody [Ret40f] recognizes Glycophorin C
Tested Reactivity Hu
Tested Application IHC-Fr, IHC-P
Host Mouse
Clonality Monoclonal
Clone Ret40f
Isotype IgG1, kappa
Target Name Glycophorin C
Immunogen Red cell ghosts
Conjugation Un-conjugated
Alternate Names Glycophorin-D; CD236R; GPD; Glycophorin-C; Glycoprotein beta; Glycoconnectin; GPC; PAS-2; CD antigen CD236; GE; CD236; Sialoglycoprotein D; GYPD; PAS-2'

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1:25 - 1:100 in an ABC method.
IHC-P1:25 - 1:100
Application Note IHC-P: Antigen Retrieval: Heat tissue section in Sodium citrate buffer (pH 6.0) or EDTA buffer (pH 8.0)
IHC-Fr: Incubate for 30-60 minutes at room temperature is highly recommended.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2995 Human GYPC

Swiss-port # P04921 Human Glycophorin-C

Gene Symbol GYPC
Gene Full Name glycophorin C (Gerbich blood group)
Background Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
Function This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. [UniProt]
Cellular Localization Cell membrane
Research Area Cell Biology and Cellular Response antibody
Calculated MW 14 kDa
PTM O-glycosylated with core 1 or possibly core 8 glycans.