ARG56020
anti-GnRHR / GnRH Receptor antibody [F1G4]
anti-GnRHR / GnRH Receptor antibody [F1G4] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Monkey
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [F1G4 ] recognizes GnRHR / GnRH Receptor |
|---|---|
| Tested Reactivity | Hu, Rat, Mk |
| Predict Reactivity | Pig, Rb |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | F1G4 |
| Isotype | IgG1, kappa |
| Target Name | GnRHR / GnRH Receptor |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 1-29 of Human GnRHR. (MANSASPEQNQHCSAINNSIPLMQGNLPY) |
| Conjugation | Un-conjugated |
| Alternate Names | GRHR; HH7; LRHR; LHRHR; Gonadotropin-releasing hormone receptor; GnRH receptor; GNRHR1; GnRH-R |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Tris with 1 mM EDTA (pH 9.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30968 Human Gonadotropin-releasing hormone receptor Swiss-port # P30969 Rat Gonadotropin-releasing hormone receptor |
|---|---|
| Gene Symbol | GNRHR |
| Gene Full Name | gonadotropin-releasing hormone receptor |
| Background | This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 may act as an inhibitor of GnRH-R signaling. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Calculated MW | 38 kDa |
Specific References
GnRH receptor expression and endometrial cell proliferation of Macaca nemestrina after administration of GnRH agonist in controlled ovarian stimulation procedures.
IHC-P / Monkey
