ARG11035

anti-Granzyme B antibody [B18.1]

anti-Granzyme B antibody [B18.1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

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Overview

Product Description Mouse Monoclonal antibody [B18.1] recognizes Granzyme B
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone B18.1
Isotype IgG2a
Target Name Granzyme B
Antigen Species Human
Immunogen Recombinant protein corresponding to aa. 7-227 of Human Granzyme B.
Conjugation Un-conjugated
Alternate Names EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Purity > 95% (by SDS-PAGE)
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3002 Human GZMB

Swiss-port # P10144 Human Granzyme B

Gene Symbol GZMB
Gene Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Background Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
Function Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt]
Highlight Related products:
Granzyme B antibodies; Granzyme B ELISA Kits; Anti-Mouse IgG secondary antibodies;
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Calculated MW 28 kDa

Specific References

Combination of FAK inhibitor and cytokine-induced killer cell therapy: An alternative therapeutic strategy for patients with triple-negative breast cancer

IHC-P, WB / Human, Mouse

Cheng-Che Wu et al.
Biomedicine & Pharmacotherapy,  (2023)

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