ARG22617

anti-Granzyme B antibody [GB11]

anti-Granzyme B antibody [GB11] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,CyTOF®-candidate and Chimpanzee,Human,Monkey,Rhesus Monkey

Overview

Product Description

Mouse Monoclonal antibody [GB11] recognizes Granzyme B
This antibody recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B.

Tested Reactivity Hu, Chimp, Mk, R. Mk
Tested Application CyTOF®-candidate, ELISA, FACS, ICC/IF, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone GB11
Isotype IgG1
Target Name Granzyme B
Antigen Species Human
Immunogen Purified Human Granzyme B.
Conjugation Un-conjugated
Alternate Names EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
ELISA2 µg/ml - 5 µg/ml
FACS1:10 - 1:100
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. Membrane permeabilisation is required for this application.
ELISA: This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with a biotin conjugated antibody as detection reagent.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer Tris buffered saline and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3002 Human GZMB

Swiss-port # P10144 Human Granzyme B

Gene Symbol GZMB
Gene Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Background Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]
Function Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt]
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Calculated MW 28 kDa