ARG55095

anti-HA tag antibody - ChIP-Grade

anti-HA tag antibody - ChIP-Grade for ChIP,ChIP-sequencing,ICC/IF,Immunoprecipitation,Radioimmunoprecipitation assay,Western blot and Other

Controls and Markers antibody; Gene Regulation antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes HA Tag - ChIP-Grade

Tested Reactivity Other
Tested Application ChIP, ChIP-seq, ICC/IF, IP, R-IP A, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HA tag
Antigen Species Others
Immunogen KLH-conjugated encoding HA tag (CYPYDVPDYAYPYDVPDYA)
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ChIP1:100
ChIP-seqAssay-dependent
ICC/IF1:10 - 1:50
IPAssay-dependent
R-IP AAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Gene Regulation antibody

Customer's Feedback

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Review for anti-HA tag antibody - ChIP-Grade

Application:WB

Sample:293T and 293T + myc-HA-mCherry

Sample Loading Amount:10 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Characterization of the interactions between Codanin-1 and C15Orf41, two proteins implicated in congenital dyserythropoietic anemia type I disease.

ICC/IF / Other

Swickley Grace et al.
BMC Molecular and Cell Biology.,  (2020)

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Reprogramming the endogenous type III-A CRISPR-Cas system for genome editing, RNA interference and CRISPRi screening in Mycobacterium tuberculosis.

R-IP A / Other

Rahman Khaista et al.
Preprint.,  (2020)

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