ARG62338

anti-HA tag antibody [HA.C5]

anti-HA tag antibody [HA.C5] for Dot blot,ELISA,Flow cytometry,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Gene Regulation antibody
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Overview

Product Description Mouse Monoclonal antibody [HA.C5] recognizes HA tag
Tested Reactivity Other
Tested Application Dot, ELISA, FACS, ICC/IF, IHC, IP, WB
Specificity Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins
Host Mouse
Clonality Monoclonal
Clone HA.C5
Isotype IgG3
Target Name HA tag
Antigen Species Others
Immunogen YPYDVPDYA (HA) synthetic peptide conjugated to KLH.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ELISAAssay-dependent
FACSAssay-dependent
ICC/IF1:500 - 1:2000
IHC1:500 - 1:2000
IP1:50-1:200
WB1:1000 - 1:3000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A Purified
Purification Note Protein A affinity chromatography from mouse ascites fluid.
Buffer 10mM PBS (pH 7.2) and 0.05% Sodium azide.
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Duo to HA-tag is from influenza virus and not be found in nomal anamils, it is useful for the protein functional analysis when the specific antibodies for the protein-of-interest are unavailable.
Highlight Related products:
HA tag antibodies; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Gene Regulation antibody

Customer's Feedback

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Review for anti-HA tag antibody [HA.C5]

Application:WB

Sample:293T and 293T + myc-HA-mCherry

Sample Loading Amount:10 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK

IP / 

Yang Yang et al.
Cell Death Differ.,  (2022)

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Translation initiation factor eIF4G1 modulates assembly of the polypeptide exit tunnel region in yeast ribosome biogenesis

WB / Yeast

Yun-Ting Tseng et al.
J Cell Sci,  (2022)

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