ARG66389

anti-HA tag antibody [SQab18117]

anti-HA tag antibody [SQab18117] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Other

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Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab18117] recognizes HA tag
Tested Reactivity Other
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab18117
Isotype IgG
Target Name HA tag
Antigen Species Others
Immunogen YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:400 - 1:2000
ICC/IF1:400 - 1:2000
IP1:10
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Customer's Feedback

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Review for anti-HA tag antibody [SQab18117]

Application:IHC

Sample:Frozen Mouse brain tissue section

Sample Preparation Method:PBS and 4% PFA perfusion

Fixation Buffer:4% PFA

Fixation Time:overnight

Antigen Retrieval Buffer:Citrate pH 6.0

Permeabilization Buffer:0.3% PBST (Triton x-100)

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC