ARG66474
anti-HAND1 antibody
anti-HAND1 antibody for ICC/IF,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HAND1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Bov, Pig, Rb, Sheep |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HAND1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide within the C-terminal region of Human HAND1. |
| Conjugation | Un-conjugated |
| Alternate Names | Class A basic helix-loop-helix protein 27; Thing1; bHLHa27; eHAND; Hxt; eHand; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; Heart- and neural crest derivatives-expressed protein 1 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 25 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O96004 Human Heart- and neural crest derivatives-expressed protein 1 Swiss-port # P97832 Rat Heart- and neural crest derivatives-expressed protein 1 |
|---|---|
| Gene Symbol | HAND1 |
| Gene Full Name | heart and neural crest derivatives expressed 1 |
| Background | The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008] |
| Function | Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity). [UniProt] |
| Cellular Localization | Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity (By similarity). [UniProt] |
| Calculated MW | 24 kDa |
| PTM | Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity. [UniProt] |
