ARG57450

anti-HARS antibody

anti-HARS antibody for Western blot and Human,Monkey

Overview

Product Description Mouse Monoclonal antibody recognizes HARS
Tested Reactivity Hu, Mk
Tested Application WB
Specificity This antibody detects endogenous levels of HARS and does not cross-react with related proteins.
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name HARS
Antigen Species Human
Immunogen Purified recombinant Human HARS protein fragments expressed in E. coli.
Conjugation Un-conjugated
Alternate Names HisRS; USH3B; EC 6.1.1.21; Histidyl-tRNA synthetase; Histidine-tRNA ligase, cytoplasmic; HRS

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.03% Proclin300 and 50% Glycerol.
Preservative 0.03% Proclin300
Stabilizer 50% Glycerol
Concentration 1.1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3035 Human HARS

Swiss-port # P12081 Human Histidine--tRNA ligase, cytoplasmic

Gene Symbol HARS
Gene Full Name histidyl-tRNA synthetase
Background Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Cellular Localization Cytoplasm.
Calculated MW 57 kDa