ARG57450
anti-HARS antibody
anti-HARS antibody for Western blot and Human,Monkey
Overview
| Product Description | Mouse Monoclonal antibody recognizes HARS |
|---|---|
| Tested Reactivity | Hu, Mk |
| Tested Application | WB |
| Specificity | This antibody detects endogenous levels of HARS and does not cross-react with related proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | HARS |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human HARS protein fragments expressed in E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | HisRS; USH3B; EC 6.1.1.21; Histidyl-tRNA synthetase; Histidine-tRNA ligase, cytoplasmic; HRS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 0.03% Proclin300 and 50% Glycerol. |
| Preservative | 0.03% Proclin300 |
| Stabilizer | 50% Glycerol |
| Concentration | 1.1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12081 Human Histidine--tRNA ligase, cytoplasmic |
|---|---|
| Gene Symbol | HARS |
| Gene Full Name | histidyl-tRNA synthetase |
| Background | Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| Cellular Localization | Cytoplasm. |
| Calculated MW | 57 kDa |
