ARG57756

anti-HCLS1 antibody

anti-HCLS1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes HCLS1
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HCLS1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-280 of Human HCLS1 (NP_005326.2).
Conjugation Un-conjugated
Alternate Names p75; LckBP1; Hematopoietic cell-specific LYN substrate 1; HS1; Hematopoietic lineage cell-specific protein; CTTNL; lckBP1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 15163 Mouse HCLS1

GeneID: 3059 Human HCLS1

Swiss-port # P14317 Human Hematopoietic lineage cell-specific protein

Swiss-port # P49710 Mouse Hematopoietic lineage cell-specific protein

Gene Symbol HCLS1
Gene Full Name hematopoietic cell-specific Lyn substrate 1
Function Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. [UniProt]
Cellular Localization Cytoplasm, Membrane, Mitochondrion, Peripheral membrane protein. [UniProt]
Calculated MW 54 kDa
PTM Phosphorylated by FES (By similarity). Phosphorylated by LYN, FYN and FGR after cross-linking of surface IgM on B-cells. Phosphorylation by LYN, FYN and FGR requires prior phosphorylation by SYK or FES. [UniProt]