ARG41383

anti-HEC1 antibody

anti-HEC1 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes HEC1
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HEC1
Antigen Species Human
Immunogen Synthetic peptide derived from Human HEC1.
Conjugation Un-conjugated
Alternate Names Kinetochore-associated protein 2; TID3; hsNDC80; HEC1; KNTC2; Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; HEC; Retinoblastoma-associated protein HEC; HsHec1; Kinetochore protein Hec1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 75 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10403 Human NDC80

GeneID: 67052 Mouse NDC80

Swiss-port # O14777 Human Kinetochore protein NDC80 homolog

Swiss-port # Q9D0F1 Mouse Kinetochore protein NDC80 homolog

Gene Symbol NDC80
Gene Full Name NDC80 kinetochore complex component
Background This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
Function Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. [UniProt]
Cellular Localization Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. [UniProt]
Calculated MW 74 kDa
PTM Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB. [UniProt]