ARG43091

anti-HECTD3 antibody

anti-HECTD3 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes HECTD3
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name HECTD3
Antigen Species Human
Immunogen Synthetic peptide corresponding to a sequence of Human HECTD3. (HYASAKVCEEKLRYAAYNCVAIDTDMSPWEE)
Conjugation Un-conjugated
Alternate Names E3 ubiquitin-protein ligase HECTD3; EC 6.3.2.-; HECT domain-containing protein 3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Caco-2, Rat brain and Mouse brain
Observed Size ~ 97 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 76608 Mouse HECTD3

GeneID: 79654 Human HECTD3

Swiss-port # Q3U487 Mouse E3 ubiquitin-protein ligase HECTD3

Swiss-port # Q5T447 Human E3 ubiquitin-protein ligase HECTD3

Gene Symbol HECTD3
Gene Full Name HECT domain containing E3 ubiquitin protein ligase 3
Background The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8. [provided by RefSeq, Dec 2012]
Function E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus facilitating cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity). [UniProt]
Cellular Localization Cytoplasm, perinuclear region. [UniProt]
Calculated MW 97 kDa