ARG57022

anti-HEXA antibody [20F1]

anti-HEXA antibody [20F1] for Western blot,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [20F1] recognizes HEXA
Tested Reactivity Hu
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 20F1
Isotype IgG2a, lambda
Target Name HEXA
Antigen Species Human
Immunogen Recombinant fragment around aa. 89-529 of Human HEXA.
Conjugation Un-conjugated
Alternate Names N-acetyl-beta-glucosaminidase subunit alpha; Beta-N-acetylhexosaminidase subunit alpha; EC 3.2.1.52; TSD; Beta-hexosaminidase subunit alpha; Hexosaminidase subunit A

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WB1:3000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3073 Human HEXA

Swiss-port # P06865 Human Beta-hexosaminidase subunit alpha

Gene Symbol HEXA
Gene Full Name hexosaminidase A (alpha polypeptide)
Background This gene encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I). [provided by RefSeq, Jul 2009]
Function Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. [UniProt]
Calculated MW 61 kDa
PTM N-linked glycan at Asn-115 consists of Man(3)-GlcNAc(2).