ARG62511

anti-HGF antibody [4C12.1]

anti-HGF antibody [4C12.1] for ELISA and Human

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [4C12.1] recognizes HGF
Tested Reactivity Hu
Tested Application ELISA
Host Mouse
Clonality Monoclonal
Clone 4C12.1
Isotype IgG2b
Target Name HGF
Antigen Species Human
Immunogen Recombinant human HGF protein.
Conjugation Un-conjugated
Alternate Names HPTA; Scatter factor; F-TCF; Hepatocyte growth factor; DFNB39; Hepatopoietin-A; HGFB; SF

Application Instructions

Application Note ELISA: 5 µg/ml
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A purified
Buffer PBS
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3082 Human HGF

Swiss-port # P14210 Human Hepatocyte growth factor

Gene Symbol HGF
Gene Full Name hepatocyte growth factor (hepapoietin A; scatter factor)
Background Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. [UniProt]
Cellular Localization Cytoplasmic
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 83 kDa

Clone References

In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor.

WB / Dog

Arends B et al.
Vet J.,  (2008)

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