ARG41508
anti-HIF-1 beta antibody
anti-HIF-1 beta antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HIF-1 beta |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HIF-1 beta |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human HIF-1 beta. |
| Conjugation | Un-conjugated |
| Alternate Names | HIF1BETA; Hypoxia-inducible factor 1-beta; Aryl hydrocarbon receptor nuclear translocator; HIF-1-beta; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; HIF1B; TANGO; HIF1-beta; bHLHe2; ARNT protein; HIF-1beta |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human fetal kidney | ||||||
| Observed Size | ~ 87 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P27540 Human Aryl hydrocarbon receptor nuclear translocator |
|---|---|
| Gene Symbol | ARNT |
| Gene Full Name | aryl hydrocarbon receptor nuclear translocator |
| Background | This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] |
| Function | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 87 kDa |
