ARG23121

anti-HLA A2 antibody [BB7.2] (FITC)

anti-HLA A2 antibody [BB7.2] (FITC) for Flow cytometry and Human

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [BB7.2] recognizes HLA A2
Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.
Tested Reactivity Hu
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone BB7.2
Isotype IgG2b
Target Name HLA A2
Antigen Species Human
Immunogen Papain solubilised HLA A2.
Conjugation FITC
Alternate Names MHC class I antigen A*1; HLAA; HLA class I histocompatibility antigen, A-1 alpha chain

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:10
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide and 1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3105 Human HLA-A

Swiss-port # P30443 Human HLA class I histocompatibility antigen, A-1 alpha chain

Gene Symbol HLA-A
Gene Full Name major histocompatibility complex, class I, A
Background HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
Function Involved in the presentation of foreign antigens to the immune system. [UniProt]
Calculated MW 40 kDa
PTM Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity). [UniProt]